Composite

Part:BBa_K2611010:Design

Designed by: Qinling Qiu   Group: iGEM18_SCU-China   (2018-10-17)


sgRNA(spacer J23101-GFP)-spacer J23101-GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 226
    Illegal NheI site found at 249
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 924


Design Notes

Carefully notice the complementary of the gRNA and its target sequence and the spacer sequence and the NGG site should be close to the promoter.(no space in this part).


Source

This part consists of two parts we submit this year, which are BBa_K2611000 and BBa_K2611001.


References