Composite
Part:BBa_K2611010:Design
Designed by: Qinling Qiu Group: iGEM18_SCU-China (2018-10-17)
sgRNA(spacer J23101-GFP)-spacer J23101-GFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 226
Illegal NheI site found at 249 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 924
Design Notes
Carefully notice the complementary of the gRNA and its target sequence and the spacer sequence and the NGG site should be close to the promoter.(no space in this part).
Source
This part consists of two parts we submit this year, which are BBa_K2611000 and BBa_K2611001.